Incucyte® Caspase-3/7 Green Reagent from Sartorius

Product Specs
ItemIncucyte® Caspase-3/7 Green Reagent
CompanySartorius
Price Supplier Page View Company Product Page
Catalog Number4440
Quantity1 vial
ApplicationsApoptosis, Cell Imaging
TypeReagent

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Local Office

Sartorius

5 Orville Drive

Bohemia, NY 11716

United States

Company Profile

International Office

Sartorius

Otto-Brenner-Straße 20

37079 Göttingen

Germany

Phone: +49 551 308 0

Company Profile

Website: www.sartorius.com

(1) PARP Inhibitors Effectively Reduce MAPK Inhibitor Resistant Melanoma Cell Growth and Synergize with MAPK Inhibitors through a Synthetic Lethal Interaction In Vitro and In VivoCancer Research CommunicationsSeptember 5, 2023Lisa Marie Fröhlich, Heike Niessner, Birgit Sauer, Sofie Kämereit, Eftychia Chatziioannou, Simon Riel, Tobias Sinnberg, Birgit Schittekdata. Apoptosis Assay Apoptosis assay was performed with the Incucyte Caspase-3/7 Dye for apoptosis (Sartorius 4440) according to the manufacturer's protocol. Briefly, 5 to 10 × 103 cells were seeded into 96-well plate

(2) A target discovery pipeline identified ILT3 as a target for immunotherapy of multiple myelomaCell reports. MedicineJuly 18, 2023Di Meo F, Iyer A, Akama K, Cheng R, Yu C, Cesarano A, Kurihara N, Tenshin H, Aljoufi A, Marino S, et al.IS,Miltenyi,130-097-743 CellTrace™ CFSE Cell Proliferation Kit,Thermo Fisher,C34554 Caspase 3/7 Green Reagent,Sartorius,4440 magnetic beads,Miltenyi,130-051-301 ,, Recombinant DNA,Recombinant DNA,Recombinant DNA ,, pCW-Cas9,Addgene,50661 pLX-sgRNA,Addgene,50662 Nuclight

(3) Antibody-dependent cellular cytotoxicity-inducing antibodies enhance the natural killer cell anti-cancer response against patient-derived pancreatic cancer organoidsFrontiers in ImmunologyJuly 14, 2023Nicky A. Beelen, Merel R. Aberle, Virginia Bruno, Steven W. M. Olde Damink, Gerard M. J. Bos, Sander S. Rensen, Lotte Wietento reach a final concentration of 10%. To quantify apoptosis, Caspase-3/7 dye (Sartorius, Cat. No. 4440) was added to each well at a final concentration of 2.5 µM. The NK cells and Caspase-3/7 dye were added

(4) Alanine supplementation exploits glutamine dependency induced by SMARCA4/2-lossNature CommunicationsMay 20, 2023Xianbing Zhu, Zheng Fu, Shary Y. Chen, Dionzie Ong, Giulio Aceto, Rebecca Ho, Jutta Steinberger, Anie Monast, Virginie Pilon, Eunice Li, et al.Dimethyl-α-ketoglutarate (DMKG, #349631) was from Sigma (Oakville, ON, Canada). Caspase3/7 green dye (4440) was from Sartorius (Goettingen, Germany). Antibodies against HSP90 (H-114, 1:1,0000) and β-Actin (Cat#

(5) Large libraries of single-chain trimer peptide-MHCs enable antigen-specific CD8+ T cell discovery and analysisCommunications BiologyMay 16, 2023William Chour, Jongchan Choi, Jingyi Xie, Mary E. Chaffee, Thomas M. Schmitt, Kathryn Finton, Diana C. DeLucia, Alexander M. Xu, Yapeng Su, Daniel G. Chen, et al.microlitres of CTL media containing CD28 antibody (100 ng/mL) and Caspase-3/7 dye (5 µM, Sartorius, 4440) was added into the well of a 96-well plate. 50 k of peptide-pulsed T2 target cells and unlabeled 100

(6) TSPO acts as an immune resistance gene involved in the T cell mediated immune control of glioblastomaActa neuropathologica communicationsMay 8, 2023Menevse AN, Ammer LM, Vollmann-Zwerenz A, Kupczyk M, Lorenz J, Weidner L, Hussein A, Sax J, Mühlbauer J, Heuschneider N, et al.(PeproTech, #310–04). Afterwards, Incucyte® Caspase-3/7 Green Dye was added to cells (1:1000, Sartorius, #4440). Cells were imaged for the indicated time points at a 10x magnification. Tumor cell apoptosis was quantified

(7) Inhibition of PLK1 Destabilizes EGFR and Sensitizes EGFR-Mutated Lung Cancer Cells to Small Molecule Inhibitor OsimertinibCancers (Basel)May 2, 2023Carolien Eggermont, Gustavo J. Gutierrez, Jacques De Grève, Philippe Gironwith the compounds, the Incucyte® Annexin V (4641, Sartorius, Göttingen, Germany) and Caspase-3/7 (4440, Sartorius) dyes were added to the cells according to the manufacturer’s protocol. To monitor Caspase-3/7

(8) CDK4/6 inhibition enhances SHP2 inhibitor efficacy and is dependent upon restoration of RB function in malignant peripheral nerve sheath tumorsbioRxiv : the preprint server for biologyFebruary 3, 2023Wang J, Calizo A, Zhang L, Pino JC, Lyu Y, Pollard K, Zhang X, Larsson AT, Conniff E, Llosa N, et al.96-well plates and treated with the indicated dose of drugs or caspase-3/7 dye for apoptosis (Sartorius, #4440). Cell proliferation and apoptosis were monitored by a microscope gantry that was connected to a network

(9) INBRX-120, a CD8α-targeted detuned IL-2 that selectively expands and activates tumoricidal effector cells for safe and durable in vivo responsesJournal for ImmunoTherapy of CancerJanuary 18, 2023Florian J Sulzmaier, Nadja Kern, Sae Jeong Ahn, Anya Polovina, Jason Ho, Abrahim Hussain, Garrett Cyprus, Chelsie Macedo, Rajay Pandit, William Crago, et al.T cells to a defined effector-to-target ratio (optimal 10:1). Incucyte Caspase-3/7 dye (Sartorius, #4440) was added to the co-cultures to determine target cell apoptosis induced by NK cells or CD8 T cells.

(10) BCL2L11 Induction Mediates Sensitivity to Src and MEK1/2 Inhibition in Thyroid CancerCancersJanuary 6, 2023Rose MM, Espinoza VL, Hoff KJ, Pike LA, Sharma V, Hofmann MC, Tan AC, Pozdeyev N, Schweppe RE.with 50 nM dasatinib, 100 nM trametinib, alone or in combination, and Caspase-3/7 Dye (Sartorius Cat. 4440, Bohemia, NY, USA) for apoptosis was added for a final concentration of 5 μM. Apoptosis was measured

(11) Ribonuclease 1 Enhances Antitumor Immunity against Breast Cancer by Boosting T cell ActivationInternational journal of biological sciencesJanuary 1, 2023Wang YN, Lee HH, Jiang Z, Chan LC, Hortobagyi GN, Yu D, Hung MC.or primary T cells at a 1:10 ratio containing caspase 3/7 substrate green dye (1:1000; Sartorius, #4440), in the presence or absence of RNase1 treatment. Green fluorescent signals and phase object confluence

(12) Effects of osteoblast-derived extracellular vesicles on aggressiveness, redox status and mitochondrial bioenergetics of MNNG/HOS osteosarcoma cellsFrontiers in OncologyDecember 5, 2022Marco Ponzetti, Argia Ucci, Chiara Puri, Luca Giacchi, Irene Flati, Daria Capece, Francesca Zazzeroni, Alfredo Cappariello, Nadia Rucci, Stefano Faloneplates and starved O/N. Then, cells were stained with a caspase 3/7-sensitive probe (Sartorius, cat. no. 4440) that crosses cell membranes but is only fluorescent in cells with active caspases 3/7, along with OB-EVs

(13) Large libraries of single-chain trimer peptide-MHCs enable rapid antigen-specific CD8+ T cell discovery and analysisResearch squareNovember 18, 2022Heath J, Chour W, Choi J, Xie J, Chaffee M, Schmitt T, Finton K, Delucia D, Xu A, Su Y, et al.peptide. 100 μL of CTL media containing CD28 antibody (100 ng/mL) and Caspase-3/7 dye (5 μM, Sartorius, 4440) was added into the well of a 96-well plate. 50 k of peptide pulsed T2 target cells and unlabeled 100

(14) Extensive preclinical validation of combined RMC-4550 and LY3214996 supports clinical investigation for KRAS mutant pancreatic cancerCell Reports MedicineNovember 15, 2022Katrin J. Frank, Antonio Mulero-Sánchez, Alexandra Berninger, Laura Ruiz-Cañas, Astrid Bosma, Kıvanç Görgülü, Nan Wu, Kalliope N. Diakopoulos, Ezgi Kaya-Aksoy, Dietrich A. Ruess, et al.commercial assays ,, CellTiter-Glo 3D,Promega,REF G9682LOT 0000496328 Caspase 3/7 reagent,Sartorius,#4440 ,, Deposited data,Deposited data,Deposited data ,, Uncropped Western Blots,Mendeley,Mendeley Data:

(15) NDUFS3 knockout cancer cells and molecular docking reveal specificity and mode of action of anti-cancer respiratory complex I inhibitorsOpen BiologyNovember 9, 2022Ivana Kurelac, Beatrice Cavina, Manuela Sollazzo, Stefano Miglietta, Agnese Fornasa, Monica De Luise, Maria Iorio, Eleonora Lama, Daniele Traversa, Hamid Razi Nasiri, et al.evaluation Apoptosis upon drug treatments was determined using the Incucyte Caspase-3/7 green dye (Sartorius #4440). Cells (SKOV3, HCT116 and B16-F10) were seeded in a 96-wells plate in high glucose DMEM, as described

(16) Computational pharmacogenomic screen identifies drugs that potentiate the anti-breast cancer activity of statinsNature communicationsOctober 24, 2022van Leeuwen JE, Ba-Alawi W, Branchard E, Cruickshank J, Schormann W, Longo J, Silvester J, Gross PL, Andrews DW, Cescon DW, et al.DR51000), 10 µM TMRE (Life Technologies, T669) and 5 µM IncuCyte® Caspase-3/7 Dye for Apoptosis (Sartorius, 4440). DRAQ5 staining was used to identify and segment individual cells as well as to measure nuclear condensation,

(17) VRK1 as a synthetic lethal target in VRK2 promoterâ€“methylated cancers of the nervous systemJCI InsightOctober 10, 2022Jonathan So, Nathaniel W. Mabe, Bernhard Englinger, Kin-Hoe Chow, Sydney M. Moyer, Smitha Yerrum, Maria C. Trissal, Joana G. Graca Marques, Jason J. Kwon, Brian H Shim, et al.catalog 353047). Then 5 mM of IncuCyte Caspase-3/7 Green Apoptosis Assay Reagent (Sartorius, catalog 4440) as well as 1:500 of Nuclight Rapid Red Dye (Sartorius, catalog 4717) were added to each well. The plate

(18) SMAD2/3 mediate oncogenic effects of TGF-β in the absence of SMAD4Communications biologyOctober 7, 2022Bertrand-Chapel A, Caligaris C, Fenouil T, Savary C, Aires S, Martel S, Huchedé P, Chassot C, Chauvet V, Cardot-Ruffino V, et al.serum starved (1% FBS medium), treated or not with TGF-β and cleaved-Caspase 3/7 Green dye (Sartorius #4440) was added in the culture medium to follow apoptosis. Images were acquired and analyzed for 72 h with

(19) Multifaceted role of RNA editing in promoting loss-of-function of PODXL in canceriScienceAugust 19, 2022Ting Fu, Tracey W. Chan, Jae Hoon Bahn, Tae-Hyung Kim, Amy C. Rowat, Xinshu Xiao,Thermo Fisher Scientific,Cat# V35004 "Incucyte® Caspase 3/7 Green Dye for apoptosis, Green",Sartorius,Cat# 4440 AgeI-HF,NEB,Cat# R3552S EcoRI-HF,NEB,Cat# R3101S NotI-HF,NEB,Cat# R3189S BstBI,NEB,Cat# R0519S XbaI,NEB,Cat#

(20) Targeting MDM4 as a Novel Therapeutic Approach in Prostate Cancer Independent of p53 StatusCancers (Basel)August 16, 2022Javier Octavio Mejía-Hernández et al.iodide [PI] (5 μg/mL/well; Cat. P4864, Sigma-Aldrich), Incucyte® Caspase-3/7 Red Dye (1:1000/well, Cat. 4440, Sartorius, New York, NY, USA) and combinations of these. The concentration of DMSO (vehicle) (Cat.

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Incucyte® Caspase-3/7 Green Reagent from Sartorius

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Incucyte® Caspase-3/7 Green Reagent from Sartorius

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